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The work is devoted to the investigation of low-temperature properties of alternative jet fuels mixed with bio-additives derived from rapeseed oil, methanol and ethanol. For this work, a modification of conventional jet fuel from rapeseed oil, methanol and ethanol was chosen to develop alternative jet fuels. The main low-temperature characteristics – freezing point and pour point of conventional jet fuel and three types of bio-additives were identified and compared with the requirements for conventional Jet A-1 fuel. The influence of bio-additives from rapeseed oil, methanol and ethanol on low-temperature characteristics of new jet fuels has been studied and explained. The necessity of studying new technologies is grounded. Mathematical dependencies of freezing point and viscosity of alternative fuels on bio-additives content were proposed.